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Binding, Stability, Kinetics |
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Isothermal Titration Calorimetry (ITC) and Binding Studies Isothermal Titration Calorimetry (ITC) is the universal detector for the study of biomolecular interactions, since heat is absorbed or generated in essentially all binding events. ITC is a true in-solution method - there is no chemical tagging or immobilization of binding components. A single ITC experiment measures the binding constant (KB), stoichiometry of binding (n), and thermodynamics of binding (DH, DG). These data are used to characterize the structure, activity, and function of proteins, nucleic acids, lipids, and other biomolecules. ITC data are also used to design and optimize drugs. Differential Scanning Calorimetry (DSC) and Protein Stability A primary goal of protein engineering, rational drug design, and biopharmaceutical formulation is the development, production, and storage of stable proteins with full functionality. It is important to know how proteins fold into their biologically active states, and how these active states are stabilized. Differential Scanning Calorimetry (DSC) is used to investigate protein stability, and is combined with other biophysical methods to link thermodynamics, structure, and function. Isothermal Titration Calorimetry (ITC) and Enzyme Kinetics Biomolecular interactions and enzyme catalysis are central to all biochemical pathways. Nearly half of all drug targets are enzymes. Isothermal Titration Calorimetry (ITC) is well-established in the study of affinity of molecular interactions, and is now becoming a mainstream tool in the study of enzyme kinetics. The strength of the technique lies in the universal nature of ITC. Traditional enzyme assays utilize a probe to monitor either substrate depletion or product formation. These probes are system-dependent and must be optimized for each reaction under specific conditions. Also, the substrate may need to be modified which could interfere with the catalysis reaction. For optical methods, the experimental conditions can affect the detection system, preventing accurate measurements. This means that with traditional assay methods, many enzymes do not have practical assays. |
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High-sensitivity DSC and isothermal microcalorimetry (-20 to 120°C) with in-situ mixing possibilities |
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µDSC3 evoµDSC3 evo is the lastest generation of the most popular microcalorimeter of Setaram. Its high sensitivity and versatility makes this instrument a star in many industrial and academic research laboratories all around the world. See more. |
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High-sensitivity DSC and microcalorimetry (-45 to 120°C) |
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µDSC7 evoEquipped with the exclusive "3D-Sensor Inside" Technology, the microcalorimeter Micro DSC VII is mainly designed for the study of substances in a confined environment (denaturation, transition, gelification, reaction, etc.) with rising and falling temperatures (no outside cooling system is needed) over a wide temperature range (–45 to 120°C). See more. |
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- October 18, 2010 - October 22, 2010
MATERIAUX 2010
Nantes, FRANCE
Co-organized by more twenty learned societies, these events have been meeting opportunities between the national actors in the various fields of Materials Science.
For this 3rd edition, 14 symposiums are proposed whose:
- Civil engineering and Futur Habitat
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Marseille, FRANCE
This conference will follow the very successful 2008 conference that was held in Augsburg. The conference 2010 will be organized in Marseille at a central location overlooking the old port. The aims of MOF2010 are to address all questions concerning the community working with Metal Organic Frameworks, Metal Coordination Compounds and related materials. The spectrum of topics to be treated range from synthesis and characterization of new materials to properties of materials towards potential applications in biology/medicine, catalysis, magnetism, luminescence and sorption. Both experimental and theoretical aspects will be dealt with, along with key questions pertaining to potential industrial applications of this fascinating family of materials. - September 05, 2010 - September 10, 2010
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Annecy, FRANCE
17th International Conference on Solid Compounds of Transition Elements
SCTE 2010 will be held in Annecy September, 5-10, 2010. The conference will be jointly organized by Institut Néel, CNRS-Université Joseph Fourier, Grenoble and Institut de Chimie de la Matière Condensée de Bordeaux, CNRS- Université Bordeaux 1, Bordeaux. - August 22, 2010 - August 27, 2010
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Rotterdam, THE NETHERLANDS
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NATAS 38th Annual Conference
Houston Hall University of Pennsyvania,PA, USA
38th Annual Conference Exploring the Frontiers of Thermal Analysis, Calorimetry, and Rheology August 15 - 18, 2010 Houston Hall University of Pennsyvania
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